購買熒光標(biāo)記的Annexin V，可獲得京東購物卡一張！

Cell Meter Annexin V凋亡檢測試劑盒是美國AAT Bioquest生產(chǎn)的細(xì)胞凋亡檢測試劑盒，我們的Cell Meter 檢測試劑盒是一套用于檢測細(xì)胞生存力的工具。有多種參數(shù)可用于檢測細(xì)胞活力。該特定試劑盒旨在通過測量磷脂酰絲氨酸（PS）的轉(zhuǎn)運(yùn)來監(jiān)測細(xì)胞凋亡。在凋亡中，PS轉(zhuǎn)移到質(zhì)膜的外部小葉。磷脂酰絲氨酸在細(xì)胞表面的出現(xiàn)是細(xì)胞凋亡初始/中間階段的普遍指標(biāo)，可以在觀察到形態(tài)變化之前進(jìn)行檢測。該試劑盒使用與PS特異性結(jié)合的熒光膜聯(lián)蛋白V。已經(jīng)證明膜聯(lián)蛋白V綴合物選擇性結(jié)合PS，使用帶有FITC通道的流式細(xì)胞儀（綠色熒光）優(yōu)化了此特定的檢測試劑盒，以檢測細(xì)胞凋亡。

數(shù)據(jù)分析

在活的非凋亡細(xì)胞中，膜聯(lián)蛋白V-iFluor 488檢測非誘導(dǎo)細(xì)胞中的先天性細(xì)胞凋亡，其通常為所有細(xì)胞的2-6％。 在凋亡細(xì)胞中，膜聯(lián)蛋白V-iFluor 488與磷脂酰絲氨酸結(jié)合，磷脂酰絲氨酸位于細(xì)胞膜的外部小葉上，因此導(dǎo)致染色強(qiáng)度增加。

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