生物素-16-dUTP可以通過切口平移，隨機引發，3'-末端標記或在PCR過程中酶促摻入DNA中。數字'16'是dUTP和生物素之間的接頭主鏈中的碳原子數。接頭越長，生物素與抗生物素蛋白的相互作用就越有效。另一方面，接頭越短，dUTP更有效地摻入DNA。生物素-16-dUTP用于在各種雜交應用中生產生物素化的DNA探針，包括Southern印跡，Northern印跡，斑點印跡，固定細胞和組織。

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